ctip (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
ctip
Ctip, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ctip/CtIP+Antibody/pmc12917874-60-55-57
Average 93 stars, based on 91 article reviews
Ctip, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ctip/CtIP+Antibody/pmc12917874-60-55-57
Average 93 stars, based on 91 article reviews
ctip - by Bioz Stars,
2026-10
93/100 stars
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Western Blot:Article Title: SIRT2 Mitigates Radiation-Induced Oral Mucositis by Promoting Homologous Recombination-Mediated DNA Double-Strand Break Repair in Epithelial Stem Cells Article Snippet: Fluorescence images were visualized and captured using Zeiss LSM 800 confocal microscope and analyzed via ImageJ. .. The following antibodies were used: SIRT2 (15345-1-AP, Proteintech; 1/4000 for WB, 1/1000 for IHC and 2ug/mg lysate for IP), acetyl-tubulin (66200-1-Ig, Proteintech, 1/2000 for WB and 1/1000 for IHC), P63 (sc-25268, Santa Cruz Biotechnologies, 1/500 for IHC), GAPDH (60004-1-Ig, Proteintech, 1/10000 for WB), Rad51 (ab133534, Abcam, 1/500 for IHC), p-H2AX (ab11174, Abcam, 1/1000 for IHC), Article Title: SNF2L suppresses nascent DNA gap formation to promote DNA synthesis Article Snippet: To obtain whole-cell lysates, cell pellets were incubated on ice for 30 min in lysis buffer (50 mM Tris–Cl pH = 7.4, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM aprotinin, 1 mM leupeptin, 1 mM DTT, 1 mM PMSF) supplemented with Pierce universal nuclease and 1 mM MgCl 2 . .. After centrifugation for 30 min, supernatant was isolated and quantified prior to immunoblotting with the following antibodies: SMARCA1 (1:1000, Cell Signaling Tech, 9450), SMARCA5 (1:500, Novus Bio, NB100-55301), RECQ1 (1:500, Abcam, ab172607), MUS81 (1:500, Santa Cruz, sc-53382), Article Title: SNF2L suppresses nascent DNA gap formation to promote DNA synthesis. Article Snippet: Whole cell lysate extraction and immunoblotting To obtain whole-cell lysates, cell pellets were incubated on ice for 30 min in lysis buffer (50 mM Tris–Cl pH = 7.4, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM aprotinin, 1 mM leupeptin, 1 mM DTT, 1 mM PMSF) supplemented with Pierce universal nuclease and 1 mM MgCl 2 . .. After centrifugation for 30 min, supernatant was isolated and quantified prior to immunoblotting with the following antibodies: SMARCA1 (1:1000, Cell Signaling Tech, 9450), SMARCA5 (1:500, Novus Bio, NB100-55301), RECQ1 (1:500, Abcam, ab172607), MUS81 (1:500, Santa Cruz, sc-53382), Article Title: SIRT2 Mitigates Radiation-Induced Oral Mucositis by Promoting Homologous Recombination-Mediated DNA Double-Strand Break Repair in Epithelial Stem Cells. Article Snippet: Radiation-induced oral mucositis (RIOM) is a common and debilitating toxicity of radiotherapy for head and neck squamous cell carcinoma (HNSCC), often compromising treatment adherence and profoundly impairing patient quality of life.. Despite its significant clinical impact, there are currently no safe and well-tolerated FDA-approved therapies to prevent or treat RIOM.. The underlying mechanisms remain poorly understood but are thought to involve inflammatory responses triggered by oxidative stress and DNA damage from ionizing radiation (IR). Immunohistochemistry:Article Title: SIRT2 Mitigates Radiation-Induced Oral Mucositis by Promoting Homologous Recombination-Mediated DNA Double-Strand Break Repair in Epithelial Stem Cells Article Snippet: Fluorescence images were visualized and captured using Zeiss LSM 800 confocal microscope and analyzed via ImageJ. .. The following antibodies were used: SIRT2 (15345-1-AP, Proteintech; 1/4000 for WB, 1/1000 for IHC and 2ug/mg lysate for IP), acetyl-tubulin (66200-1-Ig, Proteintech, 1/2000 for WB and 1/1000 for IHC), P63 (sc-25268, Santa Cruz Biotechnologies, 1/500 for IHC), GAPDH (60004-1-Ig, Proteintech, 1/10000 for WB), Rad51 (ab133534, Abcam, 1/500 for IHC), p-H2AX (ab11174, Abcam, 1/1000 for IHC), Article Title: SIRT2 Mitigates Radiation-Induced Oral Mucositis by Promoting Homologous Recombination-Mediated DNA Double-Strand Break Repair in Epithelial Stem Cells. Article Snippet: Radiation-induced oral mucositis (RIOM) is a common and debilitating toxicity of radiotherapy for head and neck squamous cell carcinoma (HNSCC), often compromising treatment adherence and profoundly impairing patient quality of life.. Despite its significant clinical impact, there are currently no safe and well-tolerated FDA-approved therapies to prevent or treat RIOM.. The underlying mechanisms remain poorly understood but are thought to involve inflammatory responses triggered by oxidative stress and DNA damage from ionizing radiation (IR). Centrifugation:Article Title: SNF2L suppresses nascent DNA gap formation to promote DNA synthesis Article Snippet: To obtain whole-cell lysates, cell pellets were incubated on ice for 30 min in lysis buffer (50 mM Tris–Cl pH = 7.4, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM aprotinin, 1 mM leupeptin, 1 mM DTT, 1 mM PMSF) supplemented with Pierce universal nuclease and 1 mM MgCl 2 . .. After centrifugation for 30 min, supernatant was isolated and quantified prior to immunoblotting with the following antibodies: SMARCA1 (1:1000, Cell Signaling Tech, 9450), SMARCA5 (1:500, Novus Bio, NB100-55301), RECQ1 (1:500, Abcam, ab172607), MUS81 (1:500, Santa Cruz, sc-53382), Article Title: SNF2L suppresses nascent DNA gap formation to promote DNA synthesis. Article Snippet: Whole cell lysate extraction and immunoblotting To obtain whole-cell lysates, cell pellets were incubated on ice for 30 min in lysis buffer (50 mM Tris–Cl pH = 7.4, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM aprotinin, 1 mM leupeptin, 1 mM DTT, 1 mM PMSF) supplemented with Pierce universal nuclease and 1 mM MgCl 2 . .. After centrifugation for 30 min, supernatant was isolated and quantified prior to immunoblotting with the following antibodies: SMARCA1 (1:1000, Cell Signaling Tech, 9450), SMARCA5 (1:500, Novus Bio, NB100-55301), RECQ1 (1:500, Abcam, ab172607), MUS81 (1:500, Santa Cruz, sc-53382), Isolation:Article Title: SNF2L suppresses nascent DNA gap formation to promote DNA synthesis Article Snippet: To obtain whole-cell lysates, cell pellets were incubated on ice for 30 min in lysis buffer (50 mM Tris–Cl pH = 7.4, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM aprotinin, 1 mM leupeptin, 1 mM DTT, 1 mM PMSF) supplemented with Pierce universal nuclease and 1 mM MgCl 2 . .. After centrifugation for 30 min, supernatant was isolated and quantified prior to immunoblotting with the following antibodies: SMARCA1 (1:1000, Cell Signaling Tech, 9450), SMARCA5 (1:500, Novus Bio, NB100-55301), RECQ1 (1:500, Abcam, ab172607), MUS81 (1:500, Santa Cruz, sc-53382), Article Title: SNF2L suppresses nascent DNA gap formation to promote DNA synthesis. Article Snippet: Whole cell lysate extraction and immunoblotting To obtain whole-cell lysates, cell pellets were incubated on ice for 30 min in lysis buffer (50 mM Tris–Cl pH = 7.4, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM aprotinin, 1 mM leupeptin, 1 mM DTT, 1 mM PMSF) supplemented with Pierce universal nuclease and 1 mM MgCl 2 . .. After centrifugation for 30 min, supernatant was isolated and quantified prior to immunoblotting with the following antibodies: SMARCA1 (1:1000, Cell Signaling Tech, 9450), SMARCA5 (1:500, Novus Bio, NB100-55301), RECQ1 (1:500, Abcam, ab172607), MUS81 (1:500, Santa Cruz, sc-53382), Immunofluorescence:Article Title: HDAC4 influences the DNA damage response and counteracts senescence by assembling with HDAC1/HDAC2 to control H2BK120 acetylation and homology-directed repair. Article Snippet: .. TP53BP1 (#4937, Thermo Fisher Scientific), Article Title: HDAC4 influences the DNA damage response and counteracts senescence by assembling with HDAC1/HDAC2 to control H2BK120 acetylation and homology-directed repair Article Snippet: .. TP53BP1 (#4937, Thermo Fisher Scientific), |
